Magnolol suppressed cell migration and invasion and induced cell apoptosis via inhibition of the NF-?B signaling pathway by upregulating microRNA-129 in multiple myeloma

Multiple myeloma (MM) is incurable cancer in the blood system. Magnolol an effective component against various cancers. This study tried to investigate effect and mechanism of magnolol on MM via regulating miR-129. Human normal plasma cells (nPCs) U266 LP1 were used this study, accompanied by treatment magnolol. The miR-129 inhibitor was transfected into during experiments. Cell viability detected Counting Kit-8 assay. migration invasion tested wound healing assay Transwell And Annexin-V-FITC/PI utilized assess cell apoptosis. miR-129, miR-1271-5p, miR-342-3p, miR-124-3p expressions quantitative reverse transcription-polymerase chain reaction (qRT-PCR), western blot adopted evaluate Cyclin D1, matrix metalloprotein (MMP)-7, MMP-9, phosphorylation (p)-I?B?, p-p65, p65 protein levels. In cells, with concentration increasing, viability, migration, rates, MMP-7, MMP-9 decreased, while apoptosis rose. increased expression cells. Besides, antagonized above-mentioned partly offset magnolol-induced decrease p-I?B? p-p65 expression, as well ratio suppressed induced inhibiting NF-?B pathway activation, upregulating MM.